Quantitative Polymerase Chain Reaction Using Infrared Heating on a Microfluidic Chip

Microfluidic polymerase chain reaction

George Maltezos, Alvaro Gomez, Jiang Zhong, Frank A. Gomez, and Axel Scherer Department of Electrical Engineering, California Institute of Technology, 1200 East California Boulevard, MS 200-36 Pasadena, California 91125, USA Department of Chemistry and Biochemistry, California State University, Los Angeles, 5151 State University Drive, Los Angeles, California 90032-8202, USA Keck School of Medi...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Polymerase chain reaction in microfluidic devices.

The invention of the polymerase chain reaction (PCR) has caused a revolution in molecular biology, giving access to a method of amplifying deoxyribonucleic acid (DNA) molecules across several orders of magnitude. Since the first application of PCR in a microfluidic device was developed in 1998, an increasing number of researchers have continued the development of microfluidic PCR systems. In th...

An emulsion system based on a chip polymerase chain reaction.

In this paper we describe a novel method for detecting many DNA fragments through efficient amplification by using an emulsion system based on "on-chip" PCR instead of conventional multiplex polymerase chain reaction (PCR). During the preparation of on-chip PCR, a set of primers were immobilized on a slide and other sets were in an emulsion system. Different emulsion phase primers and other rel...

An integrated CMOS quantitative-polymerase-chain-reaction lab-on-chip for point-of-care diagnostics.

Considerable effort has recently been directed toward the miniaturization of quantitative-polymerase-chain-reaction (qPCR) instrumentation in an effort to reduce both cost and form factor for point-of-care applications. Considerable gains have been made in shrinking the required volumes of PCR reagents, but resultant prototypes retain their bench-top form factor either due to heavy heating plat...